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Specialist – Protein Structure and Proteomics (Krogan Lab, UCSF)

You will be working on a project focused on studying protein interactions and structures from endogenously tagged and purified protein complexes. This role is an excellent opportunity to learn a number of valuable, cutting-edge techniques in CRISPR-based knock-in (CRISPR-ki) genomics, cell biology, and proteomics while working with a dynamic and interdisciplinary research team. The specific role(s) of the specialist will include: 1) validating CRISPR-ki cell lines and primary cells; 2) affinity purification, chemical cross-linking, and sample preparation for mass spectrometry (MS) analysis; and 3) enzymatic and virological-based assays for functional characterization of protein components.

The Krogan Lab (http://kroganlab.ucsf.edu) is a highly collaborative laboratory focusing on the molecular networks underpinning infectious disease, carcinogenesis, and psychiatric disorders. Our ideal applicant is a problem solver with a positive attitude, excellent organizational and interpersonal skills, and the ability to learn and implement methodology efficiently.

We are hiring 2 full-time specialists for this project with qualifications from junior to full specialist (see qualifications below). Candidate’s CV and/or cover letter must state required qualifications (or if pending) upon submission. Candidates must meet all Required Qualifications by the time of hire.

Required Qualifications:
• Jr. Specialist rank: Bachelor’s degree in biological sciences or an appropriate scientific field.
• Assistant/Associate Rank: Master’s degree, or Bachelor’s degree and 5-10 years of work experience in a relevant field of biological research.
• Full Specialist rank: PhD or 10+ years of work experience in a relevant field of biological research.
• Experience in the essential molecular biology techniques of molecular cloning, PCR, and western blotting.
• Experience in cell/tissue culture.
• Able to work independently and meet deadlines; recognize and resolve problems while maintaining a high level of organization.
• Effective verbal and written communication skills to interface with a highly collaborative cross-discipline team to review and analyze results.
• Experience with Microsoft Office Word, Excel

Preferred Qualifications:
• Experience with affinity purifications of proteins.
• Experience in preparation of protein samples for mass spectrometric analysis.
• Experience with chemical cross-linking.
• Experience with peptide enrichment techniques (e.g. TiO2, click chemistry).
• Experience with CRISPR.
• Experience with mass spectrometry and/or mass spectrometry data.
• Experience with managing large datasets and/or databases.

Please apply online at https://aprecruit.ucsf.edu/JPF04392, with a CV, cover letter, and two reference contacts.

Specialists (Represented):
See Table 24B (https://www.ucop.edu/academic-personnel-programs/_files/2022-23/july-2022-salary-scales/t24-b.pdf) for the salary range for this position. A reasonable estimate for this position is $49,000-$173,900.

As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.

UC San Francisco seeks candidates whose experience, teaching, research, or community service has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.