Open date: October 15th, 2019
Last review date: Friday, Nov 15, 2019 at 11:59pm (Pacific Time)
Applications received after this date will be reviewed by the search committee if the position has not yet been filled.
Final date: Thursday, Apr 15, 2021 at 11:59pm (Pacific Time)
Applications will continue to be accepted until this date, but those received after the review date will only be considered if the position has not yet been filled.
The UCSF Liver Center (livercenter.ucsf.edu) operates several core facilities as part of its mission to support liver-related research programs at UCSF. The person who fills this position will provide high-quality technical services to clients of the Liver Center’s Cell Biology Core. S/he will implement established protocols for isolating and culturing primary cells from the whole liver. This work extends from rodent to human liver, and involves in vivo perfusion of whole organs as well as ex vivo methods employing organ perfusion and mechanical cell isolation. The incumbent will be responsible for isolating cells and then purifying crude suspensions by methods ranging from MACS to centrifugal elutriation. In addition to isolating primary liver cells as a core service, the incumbent will expand and bank a defined set of popular liver cell lines used by UCSF researchers. S/he will also work closely with the BIOS (biorepository) team at UCSF to procure human liver tissue for cell isolation and biobanking. Finally, s/he may be called upon to train researchers or team members to perform liver cell isolations. To ensure smooth operation of the core facility, the incumbent will prepare and sterilize all instruments and solutions required for cell isolation and culture; maintain all relevant equipment (perfusion pumps, centrifuges, rotors, incubators, biosafety cabinets); order and receive supplies; maintain accurate records of completed work; keep up-to-date records of the cost of all work for recharge accounting; and assist with/stewardship of relevant IACUC and CHR protocols. The employee will interact with multiple clients within and outside UCSF.
• Master’s degree in a biological science or at least 5 years of experience in the relevant specialization
• At least 3 years’ experience handling laboratory animals (rats, mice)
• At least 3 years’ experience with cell culture and sterile technique
• Demonstrated knowledge of chemistry, sufficient to prepare and pH laboratory solutions
• Working knowledge of Microsoft Excel
• Experience with small animal surgery and anesthesia
• Knowledge of cell isolation methods including differential centrifugation and MACS, and possibly FACS
• Experience with small animal procedures (e.g., gavage, tail vein injection)
Appointees in the Specialist series will be expected to engage in specialized research, professional activities and do not have teaching responsibilities. Specialists are expected to use their professional expertise to make scientific and scholarly contributions, and may participate in University and Public Service. Screening of applicants will begin immediately and will continue as needed throughout the recruitment period. Salary will be commensurate with the applicants experience and training.
UC San Francisco seeks candidates whose experience, teaching, research, or community service has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
Please apply online at https://aprecruit.ucsf.edu/JPF02723
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